The refractive index and cellular thickness information contained in the phase signal recorded by Digital Holographic Microscopy is measured independently with a dual-wavelength technique exploiting the dispersion of a dye present in the perfusion medium.
© 2008 Optical Society of America
(090.0090) Holography : Holography
(090.1760) Holography : Computer holography
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
B. Rappaz, F. Charrière, T. Colomb, C. Depeursinge, P. J. Magistretti, and P. Marquet, "Simultaneous Cell Morphometry and Refractive Index Measurement with Dual-Wavelength Digital Holographic Microscopy," in Digital Holography and Three-Dimensional Imaging, OSA Technical Digest (CD) (Optical Society of America, 2008), paper DTuB5.
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