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Conference Paper
European Conference on Biomedical Optics
Munich Germany
June 12, 2005
ISBN: 0-8194-5866-X
Imaging Tools II: Microscopy (MD)

Sensing Cellular Function and Molecular Activity in vivo Using Fluorescence Lifetime Imaging Microscopy (FLIM)

Wei Zhong, Dhruv Sud, and Mary-Ann Mycek

http://dx.doi.org/10.1364/ECBO.2005.MD1


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Abstract

We present design features and applications of a novel UV-visible-NIR FLIM system with optical sectioning, high resolution (50ps), and large temporal dynamic range (750ps-1µs) to probe cellular metabolism and detect molecular interactions in vivo.

© 2005 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

Citation
W. Zhong, D. Sud, and M. Mycek, "Sensing Cellular Function and Molecular Activity in vivo Using Fluorescence Lifetime Imaging Microscopy (FLIM)," in Novel Optical Instrumentation for Biomedical Applications II, C. Depeursinge, ed., Vol. 5864 of Proc. SPIE (Optical Society of America, 2005), paper MD1.
http://www.opticsinfobase.org/abstract.cfm?URI=ECBO-2005-MD1


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