We developed a controlled-environment FLIM system towards intracellular oxygen sensing, with a resolution of approximately 8 µM, covering the entire physiologic range (0-300 µM). We measured oxygen levels in normal and cancerous living cells.
© 2005 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
D. Sud, W. Zhong, and M. Mycek, "Measurement of Intracellular Oxygen Levels Using Fluorescence Lifetime Imaging Microscopy (FLIM)," in Photon Migration and Diffuse-Light Imaging II, K. Licha and R. Cubeddu, eds., Vol. 5859 of Proc. SPIE (Optical Society of America, 2005), paper SuC3.
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