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Fluorescence Lifetime Imaging Microscopy using a Tunable Continuum Source and a Nipkow Disk Confocal Microscope

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Abstract

Multi-beam confocal sectioned fluorescence lifetime imaging microscopy is demonstrated using a Yokogawa spinning disk. The single-photon excitation source is a supercontinuum generated from a Ti:sapphire seeded photonic crystalline fibre.

© 2005 Optical Society of America

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