Use of optical clearing agents in human dermis imaging by two photon microscopy

R. Cicchi; F. S. Pavone; D. Massi; D. D. Sampson

doi:10.1364/ECBO.2005.WF5

Confocal, Multiphoton, and Nonlinear Microscopic Imaging II
Proc. SPIE (Optica Publishing Group, 2005),
paper WF5
•https://doi.org/10.1364/ECBO.2005.WF5

Use of optical clearing agents in human dermis imaging by two photon microscopy

R. Cicchi, F. S. Pavone, D. Massi, and D. D. Sampson

European Conference on Biomedical Optics 2005

Munich Germany
12–16 June 2005
ISBN: 0-8194-5862-7

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Confocal, Multiphoton and Biomedical Applications (WF)

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R. Cicchi, F. S. Pavone, D. Massi, and D. D. Sampson, "Use of optical clearing agents in human dermis imaging by two photon microscopy," in Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, T. Wilson, ed., Vol. 5860 of Proc. SPIE (Optica Publishing Group, 2005), paper WF5.

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Abstract

We investigate the application of optical clearing agents to improve the image contrast in two-photon microscopy of human dermis. Results obtained with glycerol, propylene glycol and glucose in aqueous solution are presented.

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