We investigate the application of optical clearing agents to improve the image contrast in two-photon microscopy of human dermis. Results obtained with glycerol, propylene glycol and glucose in aqueous solution are presented.
© 2005 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(190.0190) Nonlinear optics : Nonlinear optics
(190.4180) Nonlinear optics : Multiphoton processes
R. Cicchi, F. S. Pavone, D. Massi, and D. D. Sampson, "Use of Optical Clearing Agents in Human Dermis Imaging by Two Photon Microscopy," in Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, T. Wilson, ed., Vol. 5860 of Proc. SPIE (Optical Society of America, 2005), paper WF5.
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