A Real-Time PCR machine with fluorescence spectrometer and thermal cycler was built to perform DNA quantification. Besides, an analytical model was developed for predicting fluorescence. This scheme is as accurate as the commercial quantification system.
© 2005 Optical Society of America
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
P. Chen, D. Lee, J. Chien, and M. Wu, "Development of a Novel Real-Time PCR Machine," in Novel Optical Instrumentation for Biomedical Applications II, C. Depeursinge, ed., Vol. 5864 of Proc. SPIE (Optical Society of America, 2005), paper WG7.
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