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Conference Paper
European Conference on Biomedical Optics
Munich Germany
June 12, 2005
ISBN: 0-8194-5864-3
Optical Biosensing Methods and Assays (WI)

Development of a Homogeneous Assay Format for p53 Antibodies Using Fluorescence Correlation Spectroscopy

Hannes Neuweiler, Silvia Scheffler, and Markus Sauer

http://dx.doi.org/10.1364/ECBO.2005.WI4


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Abstract

Fluorescently modified peptide epitopes are applied for detection of p53 antibodies in homogeneous solution. Fluorescence correlation spectroscopy enables simultaneous detection of altered diffusional mobility and confinement of conformational flexibility of the epitopes upon antibody binding.

© 2005 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(180.0180) Microscopy : Microscopy
(180.1790) Microscopy : Confocal microscopy

Citation
H. Neuweiler, S. Scheffler, and M. Sauer, "Development of a Homogeneous Assay Format for p53 Antibodies Using Fluorescence Correlation Spectroscopy," in Diagnostic Optical Spectroscopy in Biomedicine III, A. Mycek, ed., Vol. 5862 of Proc. SPIE (Optical Society of America, 2005), paper WI4.
http://www.opticsinfobase.org/abstract.cfm?URI=ECBO-2005-WI4


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