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Simultaneous two-photon optical imaging and optical stimulation of neocortical circuits

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Abstract

We are developing a new approach to circuit studies which combines optical stimulation of individual neurons with real-time monitoring of action potential activity across neuronal networks by two-photon (2P) fluorescence Ca2+-imaging. We optically stimulate individual neocortical neurons or groups of neurons in a systematic fashion by 2P uncaging of neurotransmitters near neurons of interest. A custom-laser scanning microscope system allows us to utilize one laser for fast 2P Ca2+-imaging and 2P uncaging. By stimulating action potentials in individual neurons and by monitoring the activity of the remainder of the network it should be possible to reveal specific connections and track microcircuits.

© 2003 Optical Society of America

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