Abstract

Previous studies [1,2] demonstrated the computed tomography imaging spectrometer’s (CTIS) usefulness in the observation of individual cellular responses across an extended field of view (FOV) and than detection of more complicated signaling cascades. In this paper, we present modified CTIS with optical sectioning capability to further advance applicability of the system. Tissue sections from nearly all organs are comprised of multiple cell types with unique functional characteristics. The problem with such samples are that they are composed of multiple cell layers, and therefore a significant blurring of fluorescence output light is observed with standard optics. This not only presents problems with identification of individual cell types within the sample, but also degrades the accuracy of the spectral deconvolution due to dominance of high frequency information. Therefore sectioning capability may significantly improve tissue research. It will allow to isolate cells within selected layer, increase identification rate and improve accuracy of the spectrum reconstruction.

© 2003 Optical Society of America