A two-beam, two-channel, two-photon flow cytometer is developed for both ex vivo measurement of cellular parameters and in vivo detection of fluorescence labeled cells in live animals. Real-time detection of fluorescent microbeads in the vasculature of mouse ear demonstrates the ability to do flow cytometry in vivo.
© 2004 Optical Society of America
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(190.4180) Nonlinear optics : Multiphoton processes
C. F. Zhong, J. Y. Ye, A. Myc, Z. Cao, J. Kukowska, J. R. Baker, and T. B. Norris, "In vivo flow cytometry," in Frontiers in Optics 2004/Laser Science XXII/Diffractive Optics and Micro-Optics/Optical Fabrication and Testing, OSA Technical Digest Series (Optical Society of America, 2004), paper FTuE5.
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