Fluorescence anisotropy is a well known but under-explored area in biomedical imaging. We have implemented fluorescence anisotropy imaging in the context of a confocal microscope and are exploring several interesting applications including enzyme activity imaging, determination of photosensitizer orientation in membranes, and suppression of cell and tissue autofluorescence.
© 2004 Optical Society of America
C. E. Bigelow and T. H. Foster, "Biological applications of confocal fluorescence anisotropy imaging," in Frontiers in Optics 2004/Laser Science XXII/Diffractive Optics and Micro-Optics/Optical Fabrication and Testing, OSA Technical Digest Series (Optical Society of America, 2004), paper FTuR6.
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