A Titanium-sapphire laser emitting 100fs short laser pulses at 800nm wavelength is employed to inject an impermeable dye (Lucifer Yellow) into single Bovine Aortic Endothelial cells. The cell morphology remains intact after this process. A new method to analyze dye uptake and evaluate membrane integrity is suggested.
© 2004 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.7160) Medical optics and biotechnology : Ultrafast technology
(320.0320) Ultrafast optics : Ultrafast optics
(320.2250) Ultrafast optics : Femtosecond phenomena
C. Peng, I. Wilke, and R. E. Palazzo, "Microinjection by femtosecond near-infrared laser pulses," in Frontiers in Optics 2004/Laser Science XXII/Diffractive Optics and Micro-Optics/Optical Fabrication and Testing, OSA Technical Digest Series (Optical Society of America, 2004), paper FWF3.
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