Due to the virtual-transition nature of higher-harmonics generation, harmonic optical microscopy provides a noninvasive mean for the study of early developmental dynamics of vertebrates without any fluorophores. The complicated development within zebrafish (Danio rerio) embryos can be observed clearly in vivo without any treatment on the live specimen.
© 2004 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.0190) Nonlinear optics : Nonlinear optics
(190.4160) Nonlinear optics : Multiharmonic generation
C. Sun, "Harmonic optical microscopy for noninvasive in vivo embryonic studies," in Frontiers in Optics 2004/Laser Science XXII/Diffractive Optics and Micro-Optics/Optical Fabrication and Testing, OSA Technical Digest Series (Optical Society of America, 2004), paper FWN3.
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