Photon antibunching correlation analysis yields an absolute measure of N from a small number of fluorescent molecules. We have combined this technique with FRET to obtain information on the numbers of proteins in DNA/protein complexes.
© 2005 Optical Society of America
S. Fore, Y. Yeh, T. Huser, R. Balhorn, and M. Cosman, "Application of Single Molecule FRET Photon-Correlation Spectroscopy to Studying DNA-Protein Interactions," in Frontiers in Optics, OSA Technical Digest Series (Optical Society of America, 2005), paper FWK6.
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