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Conference Paper
Frontiers in Optics
Rochester, New York United States
October 24-28, 2010
Microscopy II (FTuF)

Development of Quantitative Metrics for Second-Harmonic Generation Imaging of Collagen-Based Structures

Raghu Ambekar Ramachandra Rao and Kimani Toussaint, Jr.


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We discuss the application of harmonic analysis in second-harmonic generation microscopy in developing useful quantitative metrics for assessing tissue morphology. A comparison between the information content in forward and backward SHG images is also presented.

OCIS Codes
(100.0100) Image processing : Image processing
(100.2960) Image processing : Image analysis
(180.0180) Microscopy : Microscopy
(180.4315) Microscopy : Nonlinear microscopy

R. Ambekar Ramachandra Rao and K. Toussaint, Jr., "Development of Quantitative Metrics for Second-Harmonic Generation Imaging of Collagen-Based Structures," in Frontiers in Optics 2010/Laser Science XXVI, OSA Technical Digest (CD) (Optical Society of America, 2010), paper FTuF6.

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