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Full-spectrum hyperspectral fluorescence microscopy with the Image Mapping Spectrometer (IMS)

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Abstract

We present a novel full-spectrum hyperspectral fluorescence microscope using the Image Mapping Spectrometer (IMS). The microscope combines three filtering mechanisms – polarization filtering, spatial coherence filtering and datacube filtering by spectral unmixing – to replace traditional filters.

© 2011 Optical Society of America

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