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Conference Paper
Frontiers in Optics
San Jose, California United States
October 16-20, 2011
ISBN: 978-1-55752-917-6
Single Molecule Detection, Diagnostics and Therapy I (FTuH)

Super-Resolution 3D Co-Localization of Protein Superstructures and the Cellular Surface in Live Caulobacter crescentus

Matthew D. Lew, Steven F. Lee, Jerod L. Ptacin, Marissa K. Lee, Lucy Shapiro, and W.E. Moerner  »View Author Affiliations


http://dx.doi.org/10.1364/FIO.2011.FTuH2


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Abstract

We demonstrate live cell 3D super-resolution fluorescence imaging of internal protein superstructures co-localized with the cell surface. Our sequential two-color imaging technique demonstrates the advantage of using only one reading laser.

© 2011 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

Citation
M. D. Lew, S. F. Lee, J. L. Ptacin, M. K. Lee, L. Shapiro, and W. E. Moerner, "Super-Resolution 3D Co-Localization of Protein Superstructures and the Cellular Surface in Live Caulobacter crescentus," in Frontiers in Optics 2011/Laser Science XXVII, OSA Technical Digest (Optical Society of America, 2011), paper FTuH2.
http://www.opticsinfobase.org/abstract.cfm?URI=FiO-2011-FTuH2


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