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Conference Paper
Frontiers in Optics
San Jose, California United States
October 16-20, 2011
ISBN: 978-1-55752-917-6
Single Molecule Detection, Diagnostics and Therapy II (FTuO)

Studying Subunit Cooperativity by Counting Hydrolyzed ATP on Single Chaperonin Nanomachines in Solution

Yan Jiang, Nick Douglas, Nick Conley, Erik Miller, Judith Frydman, and W.E. Moerner  »View Author Affiliations


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Single chaperonin proteins are trapped by an Anti-Brownian Electrokinetic trap and hydrolyzed Cy3-ATP counted using stepwise photobleaching. Unlike ensemble averaging the observed ATP number distributions depart from the standard cooperativity models

© 2011 OSA

OCIS Codes
(020.7010) Atomic and molecular physics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy

Y. Jiang, N. Douglas, N. Conley, E. Miller, J. Frydman, and W. E. Moerner, "Studying Subunit Cooperativity by Counting Hydrolyzed ATP on Single Chaperonin Nanomachines in Solution," in Frontiers in Optics 2011/Laser Science XXVII, OSA Technical Digest (Optical Society of America, 2011), paper FTuO6.

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