Annular apertures have been explored with second-harmonic microscopy to visualize collagen fibrils in post-mortem porcine corneas. Used with the incident beam a significant visibility increase can be obtained corresponding to a larger effective numerical aperture.
© 2012 OSA
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
B. Vohnsen and J. Li, "Second-Harmonic Microscopy with Annular Beams: Enhancement of Cornea Collagen Fibrils," in Frontiers in Optics 2012/Laser Science XXVIII, OSA Technical Digest (online) (Optical Society of America, 2012), paper FW4C.2.
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