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Conference Paper
Frontiers in Optics
Rochester, New York United States
October 14-18, 2012
ISBN: 978-1-55752-956-5
High-resolution Imaging of the Living Cornea (FW4C)

Second-Harmonic Microscopy with Annular Beams: Enhancement of Cornea Collagen Fibrils

Brian Vohnsen and JiaJun Li  »View Author Affiliations


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Annular apertures have been explored with second-harmonic microscopy to visualize collagen fibrils in post-mortem porcine corneas. Used with the incident beam a significant visibility increase can be obtained corresponding to a larger effective numerical aperture.

© 2012 OSA

OCIS Codes
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics

B. Vohnsen and J. Li, "Second-Harmonic Microscopy with Annular Beams: Enhancement of Cornea Collagen Fibrils," in Frontiers in Optics 2012/Laser Science XXVIII, OSA Technical Digest (online) (Optical Society of America, 2012), paper FW4C.2.

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