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Second-Harmonic Microscopy with Annular Beams: Enhancement of Cornea Collagen Fibrils

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Abstract

Annular apertures have been explored with second-harmonic microscopy to visualize collagen fibrils in post-mortem porcine corneas. Used with the incident beam a significant visibility increase can be obtained corresponding to a larger effective numerical aperture.

© 2012 Optical Society of America

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