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Conference Paper
Frontiers in Optics
Orlando, Florida United States
October 6-10, 2013
ISBN: 978-1-55752-987-9
Experimental Methods for Tissue Imaging and Therapy (FW4A)

Structured Laser Scanning Two-photon Superresolution Image with Temporal Intensity Modulation

Szu-Yu Chen and Chia-Hua Yeh  »View Author Affiliations


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To improve the lateral resolution of two-photon fluorescence microscopy (TPFM), the concept of structured illumination was applied by temporally modulating the excitation intensity. In this research, a 1.66-fold resolution improvement was experimentally demonstrated.

© 2013 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

S. Chen and C. Yeh, "Structured Laser Scanning Two-photon Superresolution Image with Temporal Intensity Modulation," in Frontiers in Optics 2013, I. Kang, D. Reitze, N. Alic, and D. Hagan, eds., OSA Technical Digest (online) (Optical Society of America, 2013), paper FW4A.4.

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