To improve the lateral resolution of two-photon fluorescence microscopy (TPFM), the concept of structured illumination was applied by temporally modulating the excitation intensity. In this research, a 1.66-fold resolution improvement was experimentally demonstrated.
© 2013 OSA
S. Chen and C. Yeh, "Structured Laser Scanning Two-photon Superresolution Image with Temporal Intensity Modulation," in Frontiers in Optics 2013, I. Kang, D. Reitze, N. Alic, and D. Hagan, eds., OSA Technical Digest (online) (Optical Society of America, 2013), paper FW4A.4.
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