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Conference Paper
Frontiers in Optics
Orlando, Florida United States
October 6-10, 2013
ISBN: 978-1-55752-988-6
Postdeadline Session I (FW6A)

Optical Methods for Measuring Single-Molecule Orientation and Position: Implications for Super-Resolution Microscopy

Matthew D. Lew, Mikael P. Backlund, Adam S. Backer, and W. E. Moerner  »View Author Affiliations


http://dx.doi.org/10.1364/FIO.2013.FW6A.6


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Abstract

The quadrated pupil and double-helix PSF are modifications of epifluorescence microscopes that enable precise measurements of the single-molecule orientation. We explore how position estimation beyond the diffraction limit is affected by single-molecule orientation within cells.

© 2013 OSA

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

Citation
M. D. Lew, M. P. Backlund, A. S. Backer, and W. E. Moerner, "Optical Methods for Measuring Single-Molecule Orientation and Position: Implications for Super-Resolution Microscopy," in Frontiers in Optics 2013 Postdeadline, I. Kang, D. Reitze, N. Alic, and D. Hagan, eds., OSA Postdeadline Paper Digest (online) (Optical Society of America, 2013), paper FW6A.6.
http://www.opticsinfobase.org/abstract.cfm?URI=FiO-2013-FW6A.6


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