The quadrated pupil and double-helix PSF are modifications of epifluorescence microscopes that enable precise measurements of the single-molecule orientation. We explore how position estimation beyond the diffraction limit is affected by single-molecule orientation within cells.
© 2013 OSA
M. D. Lew, M. P. Backlund, A. S. Backer, and W. E. Moerner, "Optical Methods for Measuring Single-Molecule Orientation and Position: Implications for Super-Resolution Microscopy," in Frontiers in Optics 2013 Postdeadline, I. Kang, D. Reitze, N. Alic, and D. Hagan, eds., OSA Postdeadline Paper Digest (online) (Optical Society of America, 2013), paper FW6A.6.
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