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Conference Paper
International Quantum Electronics Conference
Baltimore, Maryland United States
May 31, 2009 - June 5, 2009
ISBN: 978-1-55752-869-8
Nano-Optics and Opto-Mechanics (IMG)

Three-Dimensional Super-Resolution Single-Molecule Fluorescence Imaging Using a Double-Helix Point Spread Function

Michael A. Thompson, Sri Rama. Pavani, Julie S. Biteen, Rafael Piestun, and W. E. Moerner


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Fluorescence imaging with resolution ten times better than the diffraction limit in three dimensions over a depth of field of 2 µm is demonstrated with a widefield microscope that exhibits a double-helix point spread function.

© 2009 The Optical Society

OCIS Codes
(100.0100) Image processing : Image processing
(100.6640) Image processing : Superresolution
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy

M. A. Thompson, S. R. Pavani, J. S. Biteen, R. Piestun, and W. E. Moerner, "Three-Dimensional Super-Resolution Single-Molecule Fluorescence Imaging Using a Double-Helix Point Spread Function," in Conference on Lasers and Electro-Optics/International Quantum Electronics Conference, OSA Technical Digest (CD) (Optical Society of America, 2009), paper IMG3.

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