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Conference Paper
Laser Applications to Chemical and Environmental Analysis
Annapolis, Maryland United States
February 9, 2004
ISBN: 1-55752-765-2
Bio-Optical I (with coffee break) (TuB)

Effects of fixation on cyan fluorescent protein and its fluorescence resonance energy transfer efficiency

Alex Domin, Ming Jun Lan, and Clemens F. Kaminski

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Abstract

Fixation of samples is commonly used in fluorescence microscopy of cells. We show that fixation can have dramatic effects on the fluorescence properties of cyan fluorescent protein and on its ability to engage in FRET.

© 2004 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy

Citation
A. Domin, M. J. Lan, and C. F. Kaminski, "Effects of fixation on cyan fluorescent protein and its fluorescence resonance energy transfer efficiency," in Laser Applications to Chemical and Environmental Analysis, Technical Digest (Optical Society of America, 2004), paper TuB4.
http://www.opticsinfobase.org/abstract.cfm?URI=LACEA-2004-TuB4


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