Fixation of samples is commonly used in fluorescence microscopy of cells. We show that fixation can have dramatic effects on the fluorescence properties of cyan fluorescent protein and on its ability to engage in FRET.
© 2004 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
A. Domin, M. J. Lan, and C. F. Kaminski, "Effects of fixation on cyan fluorescent protein and its fluorescence resonance energy transfer efficiency," in Laser Applications to Chemical and Environmental Analysis, Technical Digest (Optical Society of America, 2004), paper TuB4.
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