We present an approach which uses time- and spectral information to discriminate dynamic processes from concentration effects in fluorescence imaging measurements. Our instrumentation covers a TCSPC based multi-wavelength detection together with a laser scanning microscope.
© 2004 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6920) Medical optics and biotechnology : Time-resolved imaging
W. Becker and A. Bergmann, "Temporal- and spectral analysis of multi-color FLIM measurements," in Laser Applications to Chemical and Environmental Analysis, Technical Digest (Optical Society of America, 2004), paper TuC3.
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