We describe a frequency-domain fluorescence lifetime imaging (FLIM) setup that uses cheap LEDs for illumination. Application of this system is demonstrated by measuring lifetime changes caused by the diffusion of iodide ions across a microchannel.
© 2006 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
A. D. Elder, J. Swartling, S. M. Matthews, K. Yunus, J. H. Frank, and C. F. Kaminski, "Fluorescence Lifetime Imaging Using Cheap LEDs as Illumination: Application in Microchannels," in Laser Applilcations to Chemical, Security and Environmental Analysis, Technical Digest (Optical Society of America, 2006), paper TuE9.
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