Spatial resolution for depletion microscopy is estimated when radially polarized beams are used for excitation and depletion. Axial electric fields generated by focused radially polarized beams play an important role for the improvement of resolution.
© 2009 The Optical Society
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.0180) Microscopy : Microscopy
(180.4315) Microscopy : Nonlinear microscopy
S. Sato and Y. Kozawa, "Spatial Resolution for Fluorescence Depletion Microscopy Using Axial Electric Field Generated by Focused Radially Polarized Beams," in Advances in Imaging, OSA Technical Digest (CD) (Optical Society of America, 2009), paper NMA3.
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