We consider theoretical limits to microscopy techniques that involve activation and localization of sparse subsets of fluorophores. We will show that the performance of the image processing algorithm determines the trade-offs between speed and resolution.
© 2009 The Optical Society
A. R. Small, "Theoretical Limits on Speed, Errors, and Resolution in Microscopy with Switchable Fluorophores," in Advances in Imaging, OSA Technical Digest (CD) (Optical Society of America, 2009), paper NMB4.
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