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Conference Paper
Novel Techniques in Microscopy
Waikoloa Beach, Hawaii United States
April 14-18, 2013
ISBN: 978-1-55752-966-4
Super Resolution I (NM2B)

The Double-Helix Microscope Enables Precise and Accurate Measurement of 3D Single-Molecule Orientation and Localization Beyond the Diffraction Limit

Mikael Backlund, Matthew D. Lew, Adam S. Backer, Steffen J. Sahl, Ginni Grover, Anurag Agrawal, Rafael Piestun, and W. E. Moerner  »View Author Affiliations


http://dx.doi.org/10.1364/NTM.2013.NM2B.2


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Abstract

Failure to account for the asymmetric nature of fluorophore emission in single-molecule super-resolution microscopy can lead to large localization errors. We use the Double-Helix microscope to correct such mislocalizations while simultaneously extracting 3D position and molecular orientation.

© 2013 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

Citation
M. Backlund, M. D. Lew, A. S. Backer, S. J. Sahl, G. Grover, A. Agrawal, R. Piestun, and W. E. Moerner, "The Double-Helix Microscope Enables Precise and Accurate Measurement of 3D Single-Molecule Orientation and Localization Beyond the Diffraction Limit," in Optics in the Life Sciences, OSA Technical Digest (online) (Optical Society of America, 2013), paper NM2B.2.
http://www.opticsinfobase.org/abstract.cfm?URI=NTM-2013-NM2B.2


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