Failure to account for the asymmetric nature of fluorophore emission in single-molecule super-resolution microscopy can lead to large localization errors. We use the Double-Helix microscope to correct such mislocalizations while simultaneously extracting 3D position and molecular orientation.
© 2013 OSA
M. Backlund, M. D. Lew, A. S. Backer, S. J. Sahl, G. Grover, A. Agrawal, R. Piestun, and W. E. Moerner, "The Double-Helix Microscope Enables Precise and Accurate Measurement of 3D Single-Molecule Orientation and Localization Beyond the Diffraction Limit," in Optics in the Life Sciences, OSA Technical Digest (online) (Optical Society of America, 2013), paper NM2B.2.
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