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Drug response measurements using optical forces

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Abstract

A novel laser-based technology, Optophoresis™, provides quantitative analysis of cells in their native state without labeling or cell processing. Exposure of cells in an aqueous suspension to a moving optical gradient field produces forces on the cells. The measurement of the motion induced by these forces provides a quantitative basis for distinguishing one population of cells from another, e.g. by measuring the distribution of the distances that cells in a population travel when exposed to a fast moving laser beam. This technique was validated using a clinically relevant cell-based model of chronic myeloid leukemia treated with Gleevec™ (a trademark of Novartis Pharma AG) and related drugs. The measurements were quantitatively comparable to reference assays with regard to drug selectivity and potency, as well as target specificity, demonstrating the suitability of this technology for pharmaceutical and clinical applications.

© 2003 Optical Society of America

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