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Conference Paper
Optical Molecular Probes, Imaging and Drug Delivery
Waikoloa Beach, Hawaii United States
April 14-18, 2013
ISBN: 978-1-55752-966-4
Imaging Cell Function (MTh1C)

Mapping intracellular temperature using Green Fluorescent Protein - From in vitro to in vivo

jon donner, Sebastian A. Thompson, Mark P. Kreuzer, Guillaume Baffou, and Romain Quidant  »View Author Affiliations


http://dx.doi.org/10.1364/OMP.2013.MTh1C.2


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Abstract

We present the use of green fluorescent protein as a thermal nanoprobe suited for intracellular temperature mapping. In vivo and in vitro experiments show a spatial resolution of 300 nm and temperature accuracy of 0.4 C.

© 2013 OSA

OCIS Codes
(110.6820) Imaging systems : Thermal imaging
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy

Citation
j. donner, S. A. Thompson, M. P. Kreuzer, G. Baffou, and R. Quidant, "Mapping intracellular temperature using Green Fluorescent Protein - From in vitro to in vivo," in Optics in the Life Sciences, OSA Technical Digest (online) (Optical Society of America, 2013), paper MTh1C.2.
http://www.opticsinfobase.org/abstract.cfm?URI=OMP-2013-MTh1C.2


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