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Distinguishing Between Two and Three-State Equilibrium Folding with Three-Pulse Photon Echo Peak Shift (3PEPS) Spectroscopy

Zhaochuan Shen, Emily Gibson, and Ralph Jimenez

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Abstract

We investigate the equilibrium unfolding of Zn-cytochrome c by three-pulse photon echo peak shift spectroscopy. The wavelength dependence of the asymptotic peak shift reveals a bimodal inhomogeneous distribution function characteristic of two-state unfolding.

© 2010 Optical Society of America

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