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In vivo fiber-optic confocal reflectance microscope with an injection-molded plastic miniature objective lens

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Abstract

For in vivo optical diagnostic technologies to be distributed to the developed and developing worlds, optical imaging systems must be constructed of inexpensive components. We present a fiber-optic confocal reflectance microscope with a cost-effective injection-molded plastic miniature objective lens for in vivo imaging of human tissues in near real time. The measured lateral resolution is less than 2.2 µm, and the measured axial resolution is 10 µm. Confocal images of ex vivo cervical tissue biopsies and in vivo human lip taken at 15 frames/s demonstrate the microscope’s capability of imaging cell morphology and tissue architecture.

© 2005 Optical Society of America

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