Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Feedback phase correction of Bessel beams in confocal line light-sheet microscopy: a simulation study

Not Accessible

Your library or personal account may give you access

Abstract

Confocal line detection has been shown to improve contrast in light-sheet-based microscopy especially when illuminating the sample by Bessel beams. Besides their self-reconstructing capability, the stability in propagation direction of Bessel beams allows to block the unwanted emission light from the Bessel beam’s ring system. However, due to phase aberrations induced especially at the border of the specimen, Bessel beams may not propagate along lines parallel to the slit detector. Here we present a concept of how to correct the phase of each incident Bessel beam such that the efficiency of confocal line detection is improved by up to 200%–300%. The applicability of the method is verified by the results obtained from numerical simulations based on the beam propagation method.

© 2013 Optical Society of America

Full Article  |  PDF Article
More Like This
Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation

Florian O. Fahrbach, Vasily Gurchenkov, Kevin Alessandri, Pierre Nassoy, and Alexander Rohrbach
Opt. Express 21(11) 13824-13839 (2013)

Self-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy

Florian O. Fahrbach, Vasily Gurchenkov, Kevin Alessandri, Pierre Nassoy, and Alexander Rohrbach
Opt. Express 21(9) 11425-11440 (2013)

A line scanned light-sheet microscope with phase shaped self-reconstructing beams

Florian O. Fahrbach and Alexander Rohrbach
Opt. Express 18(23) 24229-24244 (2010)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (11)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (14)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved