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Optica Publishing Group
  • Chinese Optics Letters
  • Vol. 11,
  • Issue 12,
  • pp. 121102-121102
  • (2013)

Efficient autofocus method for sequential automatic capturing of high-magnification microscopic images

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Abstract

This letter presents an autofocus (AF) method to position a high-magnification microscope lens that automatically captures hundreds of images from a single moving slide. These images are taken by a mobile clinic unit in a rural location, and are later automatically processed and revised by a remote specialist. This process requires high focus precision to enable image processing techniques to achieve proper results. Low focusing times are also required for the system to be operative. We propose a novel method that combines two focus measures with an adapted searching scheme to cope with both constraints.

© 2013 Chinese Optics Letters

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