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Optica Publishing Group
  • Chinese Optics Letters
  • Vol. 12,
  • Issue 8,
  • pp. 080101-
  • (2014)

Measurement of algae PSII photosynthetic parameters using high-frequency excitation flashes

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Abstract

We establish a system to measure the functional absorption cross section of photosystem II (PSII) (σPSII) and maximum quantum yield of photochemistry in PSII (Fv/Fm). The system utilizes a sequence of high-frequency excitation flashes at microsecond intervals to induce a microsecond-level fluorescence yield curve. Parameters σPSII and Fv/Fm are calculated by fitting the curve using nonlinear regression. Experimental results show that the relative standard deviation (RSD) of the system is less than 3%, and the correlation coefficient of Fv/Fm values measured by this system and those measured by pulse amplitude modulation method is 0.950.

© 2014 Chinese Optics Letters

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