Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Image contrast enhancement in confocal ultramicroscopy

Not Accessible

Your library or personal account may give you access

Abstract

Ultramicroscopy allows for the 3D reconstruction of centimeter sized samples with a spatial resolution of several micrometers. Nevertheless, in poorly cleared or very large specimens the images may suffer from blurring and low contrast levels. To address these problems, ultramicroscopy was combined with the principle of confocal microscopy using a slowly rotating Nipkow disk. This configuration was tested by comparing images from mouse hippocampal neurons and mouse liver blood vessels recorded in confocal and conventional mode. It was found that confocality minimizes the background noise and considerably improves the signal-to-noise ratio when applied to ultramicroscopy.

© 2009 Optical Society of America

Full Article  |  PDF Article
More Like This
Feasibility of in vivo imaging of fluorescent proteins using lifetime contrast

Anand T. N. Kumar, Euiheon Chung, Scott B. Raymond, Jeroen A. J. M. van de Water, Khalid Shah, Dai Fukumura, Rakesh K. Jain, Brian J. Bacskai, and David A. Boas
Opt. Lett. 34(13) 2066-2068 (2009)

Sub-diffraction-limit imaging based on the topographic contrast of differential confocal microscopy

Chau-Hwang Lee, Hui-Yu Chiang, and Hong-Yao Mong
Opt. Lett. 28(19) 1772-1774 (2003)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved