Abstract

Spectrally encoded flow cytometry (SEFC) is a promising technique for noninvasive in vivo microscopy of blood cells. Here, we introduce a novel SEFC system for label-free confocal imaging of blood cells flowing at velocities of up to $10\mathrm{mm}/\mathrm{s}$ within 65 μm-diameter vessels. The new system employs interferometric Fourier-domain detection and a high-speed wavelength-swept source, allowing 100 kHz line rate, sufficient for sampling the rapidly flowing cells 80 μm below the tissue surface. The large data sets obtained by this technique would improve diagnosis accuracy, reduce imaging time, and open new possibilities for noninvasive monitoring of blood in patients.

© 2012 Optical Society of America

Measuring blood velocity using correlative spectrally encoded flow cytometry

Tal Elhanan and Dvir Yelin
Opt. Lett. 39(15) 4424-4426 (2014)

Noninvasive imaging of flowing blood cells using label-free spectrally encoded flow cytometry

Lior Golan, Daniella Yeheskely-Hayon, Limor Minai, Eldad J Dann, and Dvir Yelin
Biomed. Opt. Express 3(6) 1455-1464 (2012)

Measuring the red blood cell shape in capillary flow using spectrally encoded flow cytometry

Lidan Fridman and Dvir Yelin
Biomed. Opt. Express 13(9) 4583-4591 (2022)

Flow cytometry using spectrally encoded confocal microscopy

Lior Golan and Dvir Yelin
Opt. Lett. 35(13) 2218-2220 (2010)

In vitro hematocrit measurement using spectrally encoded flow cytometry

Adel Zeidan, Lior Golan, and Dvir Yelin
Biomed. Opt. Express 7(10) 4327-4334 (2016)