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Reconstructing 3-D light-microscopic images by digital image processing

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Abstract

A reconstruction procedure based on linear system theory has been developed for 3-D light-microscopic images. Inverse filtering with the 3-D optical transfer function was used for image reconstruction. The procedure allows a significant improvement in spatial resolution in the image planes perpendicular to the optical axis.

© 1985 Optical Society of America

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Three-dimensional imaging by a microscope

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J. Opt. Soc. Am. A 2(2) 121-127 (1985)

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