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Applied Optics

Applied Optics


  • Vol. 37, Iss. 10 — Apr. 1, 1998
  • pp: 1930–1934

Microlenses as amplification for CCD-based detection devices for screening applications in biology, biochemistry, and chemistry

Gregor Schlingloff, Hans-Jürgen Kiel, and Andreas Schober  »View Author Affiliations

Applied Optics, Vol. 37, Issue 10, pp. 1930-1934 (1998)

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We describe the use of microlenses as amplification for CCD-based detection devices. The possible amplification of a signal in a reaction chamber of a nanoplate is estimated with a first-order approximation. This value was proved with a commercially available microlens and with a specially constructed microlens array made of glass hemispheres. Possible applications of this approach to amplification are given.

© 1998 Optical Society of America

OCIS Codes
(040.0040) Detectors : Detectors
(170.1420) Medical optics and biotechnology : Biology
(350.3950) Other areas of optics : Micro-optics

Original Manuscript: August 21, 1997
Revised Manuscript: November 24, 1997
Published: April 1, 1998

Gregor Schlingloff, Hans-Jürgen Kiel, and Andreas Schober, "Microlenses as amplification for CCD-based detection devices for screening applications in biology, biochemistry, and chemistry," Appl. Opt. 37, 1930-1934 (1998)

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  1. J. Shimada, O. Ohguchi, R. Sawanda, “Efficient coupling device between a laser diode integrated with a microlens and an optical fiber,” in European Conference on Integrated Optics 1993, P. Roth, ed. (Neuchatel, Switzerland, 1993), pp. 11.6–11.7.
  2. W. Singer, M. Testorf, K. H. Brenner, “Gradient-index microlenses: numerical investigation of different spherical index profiles with the wave propagation method,” Appl. Opt. 34, 2165–2171 (1995). [CrossRef] [PubMed]
  3. K. Hamanaka, H. Nemoto, M. Oikawa, E. Okuda, T. Kishimoto, “Multiple imaging and Fourier transformation using planar microlenses,” Appl. Opt. 29, 4064–4070 (1990). [CrossRef] [PubMed]
  4. K. Engelhardt, K. Knop, “Passive focus sensor,” Appl. Opt. 34, 2339–2344 (1995). [CrossRef] [PubMed]
  5. A. L. Belostotsky, A. S. Leonov, “Design of aplanatic waveguide Fresnel lenses and aberration-free planar optical systems,” J. Lightwave Technol. 2, 1314–1319 (1993). [CrossRef]
  6. D. J. Arndt-Jovin, M. Robert-Nicoud, S. J. Kaufman, T. M. Jovin, “Fluorescent digital imaging microscopy in cell biology,” Science 230, 247–256 (1985). [CrossRef] [PubMed]
  7. Y. Hiraoka, D. A. Agard, J. W. Sedat, “The use of charge coupled devices for quantitative optical microscopy of biological structures,” Science 231, 36–41 (1987). [CrossRef]
  8. J. V. Sweedler, R. B. Bilhorn, P. M. Epperson, G. R. Sims, M. B. Denton, “High performance charge transfer devices,” Anal. Chem. A 40, 282–291 (1988). [CrossRef]
  9. J. A. Steinkamp, T. M. Joshida, J. C. Martin, “Flow cytometer for resolving signals from heterogeneous fluorescence emissions and quantifying life time in fluorochrome-labeled cells/particles by phase-sensitive detection,” Rev. Sci. Instrum. 64 12, 3440–3450 (1993). [CrossRef]
  10. F. Kubota, H. Kusuzawa, T. Kosaka, H. Nakamoto, “Flow cytometer and imaging device used in combination,” Cytometry 21, 129–132 (1995). [CrossRef] [PubMed]
  11. S. Herr, Th. Bastian, R. Pepperkok, Ch. Boulin, W. Ansorge, “A fully automated image acquisition and analysis system for low light level fluorescence microscopy,” Methods Mol. Cell. Biol. 4, 164–170 (1993).
  12. M. Ambroz, A. J. MacRobert, J. Morgan, G. Rumbles, M. S. C. Foley, D. Philipps, “Time-resolved fluorescence spectroscopy and intracellular imaging of disulphonated aluminum phthalocyanine,” J. Photochem. Photobiol. B 22, 105–117 (1994). [CrossRef] [PubMed]
  13. M. I. Highett, D. J. Rawlins, P. J. Shaw, “Different patterns of rDNA distribution in Pisum sativum nuclei correlate with different levels of nucleor activity,” J. Cell Sci. 104, 843–852 (1993).
  14. St. J. Hayes, St. A. Hinchliffe, J. D. Pope, P. Eccles, M. M. Khine, R. O. C. Kaschula, A. Sampedro, D. van Velzen, “Ploidy analysis on Wilms tumor touch imprints using ethidium bromide and automated image analysis integrated confocal scanning microscopy,” Virchows Arch. 427, 101–104 (1995). [CrossRef]
  15. A. R. Faruqui, H. N. Andrews, C. Raeburn, “A large cooled-CCD detector for electron microscopy,” Nucl. Instrum. Methods Phys. Res. A 348, 659–663 (1994). [CrossRef]
  16. Ch. Nicholson, L. Tao, “Hindered diffusion of high molecular weight compounds in brain extracellular microenvironment measured with integrative optical imaging,” Biophys. J. 65, 2277–2290 (1993). [CrossRef] [PubMed]
  17. E. Maier, H. R. Crollius, H. Lehrach, “Hybridization techniques on gridded high density DNA and in situ colony filters based on fluorescence detection,” Nucl. Acids Res. 22, 3423–3424 (1994). [CrossRef]
  18. Ch. Lengauer, R. M. Speicher, S. Popp, A. Jauch, M. Taniwaki, R. Nagaraja, H. C. Riethman, H. Donis-Keller, M. D’Urso, D. Schlessinger, Th. Cremer, “Chromosomal bar codes produced by multicolor fluorescence in situ hybridization with multiple YAC clones and whole chromosome painting probes,” Human Molec. Genet. 2, 505–512 (1993). [CrossRef]
  19. M. R. H. White, J. Morse, Z. A. M. Boniszewski, C. R. Mundy, M. A. W. Brady, D. J. Chriswell, “Imaging of firefly luciferase in single mammalian cells using high sensitive charge coupled device cameras,” Technique 2, 194–201 (1990).
  20. R. N. Waterhouse, L. N. Glover, “CCD monitoring of bioluminescence during the induction of cell wall-deficient, l-form state of a genetically modified strain of Pseudomonas syringae pv. phaseolicola,” Lett. Appl. Microbiol. 19, 88–91 (1994). [CrossRef] [PubMed]
  21. C. W. Earle, M. E. Baker, M. B. Denton, R. S. Pomeroy, “Imaging applications for chemical analysis utilizing charge coupled device array detectors,” Trends Analyt. Chem. 12, 395–403 (1993). [CrossRef]
  22. I. M. Warner, St. A. Soper, L. B. McGown, “Molecular fluorescence, phosphorescence and chemiluminescence spectrometry,” Anal. Chem. 68, 73R–91R (1996). [CrossRef]
  23. D. Tsi, E. Marom, J. Katz, J. Swartz, “System analysis of CCD-based bar code reader,” Appl. Opt. 32, 3504–3512 (1993). [CrossRef] [PubMed]
  24. A. Schober, J. M. Köhler, R. Günther, M. Thürk, A. Schwienhorst, J. M. Köhler, M. Eigen, M. Döring, “Parallele Führung von Reaktionen,” German patent applicationPCT/EP 94/02173 (2July1993).
  25. A. Schober, A. Schwienhorst, J. M. Köhler, M. Fuchs, R. Günther, M. Thürk, “Microsystems for independent parallel chemical and biological processing,” Microsyst. Technol. 1, 168–172 (1995). [CrossRef]
  26. J. M. Köhler, R. Pochmann, A. Schaper, A. Schober, Th. M. Jovin, A. Schwienhorst, “Micromechanical elements for detection of molecules and molecular design,” Microsyst. Technol. 1, 202–208 (1995). [CrossRef]
  27. M. Schena, D. Shalon, R. W. Davis, P. O. Brown, “Quantitative monitoring of gene expression patterns with a complementary DNA microarray,” Science 270, 467–470 (1995). [CrossRef] [PubMed]
  28. M. A. Gallop, R. W. Barrett, W. J. Dower, St. P. A. Fodor, E. M. Gordon, “Applications of combinatorial technologies to drug discovery. 1. Background and peptide combinatorial libraries,” J. Med. Chem. 37, 1233–1251 (1994). [CrossRef] [PubMed]
  29. E. M. Gordon, R. W. Barrett, W. J. Dower, St. P. A. Fodor, M. A. Gallop, “Applications of combinatorial technologies to drug discovery. 2. Combinatorial organic synthesis, library screening strategies and future directions,” J. Med. Chem. 37, 1385–1401 (1994). [CrossRef] [PubMed]
  30. M. Eigen, R. Rigler, “Sorting single molecules: application to diagnostics and evolutionary biotechnology,” Proc. Natl. Acad. Sci. USA 91, 5740–5747 (1994). [CrossRef] [PubMed]
  31. D. E. Koppel, F. Morgan, A. E. Cowan, J. H. Carson, “Scanning concentration correlation spectroscopy using the confocal laser microscope,” Biophys. J. 66, 502–507 (1994). [CrossRef] [PubMed]
  32. J. M. Köhler, G. Mayer, Th. Schulz, A. Schober, “Chip elements for combinatorial chemistry, fluid processing and PCR,” in 5th International Conference on Micro-system Technologies, H. Reichl, A. Heuberger, eds. (VDE-Verlag, Berlin, 1994), pp. 693–698.

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