For demonstrating that visible-light spectroscopy can be used for ice-front detection within freezing tissue, proton magnetic resonance images were correlated to time-evolving transmittance spectra as an ice front progressed across a tissue sample. The experimental apparatus was designed to be compatible with magnetic resonance imaging, to produce one-dimensional freezing, and to allow both reflectance and transillumination emitter–detector configurations about a normally progressing planar ice front in chicken muscle. This demonstration has potentially important medical applications in cryopreservation (freezing of biological materials for preservation) and cryosurgery (destruction of tissue by freezing).
© 1998 Optical Society of America
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6930) Medical optics and biotechnology : Tissue
(300.6550) Spectroscopy : Spectroscopy, visible
(350.5500) Other areas of optics : Propagation
David M. Otten, Boris Rubinsky, Wai-Fung Cheong, and David A. Benaron, "Ice-Front Propagation Monitoring in Tissue by the use of Visible-Light Spectroscopy," Appl. Opt. 37, 6006-6010 (1998)