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Applied Optics

Applied Optics


  • Vol. 37, Iss. 25 — Sep. 1, 1998
  • pp: 6006–6010

Ice-front propagation monitoring in tissue by the use of visible-light spectroscopy

David M. Otten, Boris Rubinsky, Wai-Fung Cheong, and David A. Benaron  »View Author Affiliations

Applied Optics, Vol. 37, Issue 25, pp. 6006-6010 (1998)

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For demonstrating that visible-light spectroscopy can be used for ice-front detection within freezing tissue, proton magnetic resonance images were correlated to time-evolving transmittance spectra as an ice front progressed across a tissue sample. The experimental apparatus was designed to be compatible with magnetic resonance imaging, to produce one-dimensional freezing, and to allow both reflectance and transillumination emitter–detector configurations about a normally progressing planar ice front in chicken muscle. This demonstration has potentially important medical applications in cryopreservation (freezing of biological materials for preservation) and cryosurgery (destruction of tissue by freezing).

© 1998 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6930) Medical optics and biotechnology : Tissue
(300.6550) Spectroscopy : Spectroscopy, visible
(350.5500) Other areas of optics : Propagation

Original Manuscript: November 3, 1997
Revised Manuscript: February 2, 1998
Published: September 1, 1998

David M. Otten, Boris Rubinsky, Wai-Fung Cheong, and David A. Benaron, "Ice-front propagation monitoring in tissue by the use of visible-light spectroscopy," Appl. Opt. 37, 6006-6010 (1998)

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