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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 37, Iss. 31 — Nov. 1, 1998
  • pp: 7410–7418

Single scattering by red blood cells

Martin Hammer, Dietrich Schweitzer, Bernhard Michel, Eike Thamm, and Achim Kolb  »View Author Affiliations


Applied Optics, Vol. 37, Issue 31, pp. 7410-7418 (1998)
http://dx.doi.org/10.1364/AO.37.007410


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Abstract

A highly diluted suspension of red blood cells (hematocrit 0.01) was illuminated with an Ar or a dye laser in the wavelength range of 458–660 nm. The extinction and the angle-resolved intensity of scattered light were measured and compared with the predictions of Mie theory, the Rayleigh–Gans approximation, and the anomalous diffraction approximation. Furthermore, empirical phase functions were fitted to the measurements. The measurements were in satisfactory agreement with the predictions of Mie theory. However, better agreement was found with the anomalous diffraction model. In the Rayleigh–Gans approximation, only small-angle scattering is described appropriately. The scattering phase function of erythrocytes may be represented by the Gegenbauer kernel phase function.

© 1998 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1530) Medical optics and biotechnology : Cell analysis
(170.7050) Medical optics and biotechnology : Turbid media
(290.0290) Scattering : Scattering
(290.5820) Scattering : Scattering measurements

History
Original Manuscript: March 11, 1998
Revised Manuscript: June 22, 1998
Published: November 1, 1998

Citation
Martin Hammer, Dietrich Schweitzer, Bernhard Michel, Eike Thamm, and Achim Kolb, "Single scattering by red blood cells," Appl. Opt. 37, 7410-7418 (1998)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-37-31-7410


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References

  1. K. Rinzema, B. J. Hoenders, H. A. Ferwerda, J. J. Ten Bosch, “Analytic calculation of the radiance in an anisotropically scattering medium close to the source,” Pure Appl. Opt. 4, 629–642 (1995). [CrossRef]
  2. M. S. Patterson, B. C. Wilson, D. R. Wyman, “The propagation of optical radiation in tissue. I. Models of radiation transport and their application,” Laser Med. Sci. 6, 155–168 (1991). [CrossRef]
  3. V. Twersky, “Absorption and multiple scattering by biological suspensions,” J. Opt. Soc. Am. 60, 1084–1093 (1970). [CrossRef] [PubMed]
  4. L. O. Reynolds, C. Johnson, A. Ishimaru, “Diffuse reflectance from a finite blood medium: applications to the modeling of fiber optic catheters,” Appl. Opt. 15, 2059–2067 (1976). [CrossRef] [PubMed]
  5. J. M. Schmitt, J. D. Meindl, F. G. Mihm, “An integrated circuit based optical sensor for in vivo measurement of blood oxygenation,” IEEE Trans. Biomed. Eng. 33, 98–107 (1986). [CrossRef] [PubMed]
  6. J. M. Steinke, A. P. Shepherd, “Diffusion model of the optical absorbance of whole blood,” J. Opt. Soc. Am. A 5, 813–822 (1988). [CrossRef] [PubMed]
  7. D. H. Tycko, M. H. Metz, E. A. Epstein, A. Grinbaum, “Flow-cytometric light scattering measurement of red blood cell volume and hemoglobin concentration,” Appl. Opt. 24, 1355–1365 (1985). [CrossRef] [PubMed]
  8. J. M. Steinke, A. P. Shepherd, “Comparison of Mie theory and the light scattering of red blood cells,” Appl. Opt. 27, 4027–4033 (1988). [CrossRef] [PubMed]
  9. A. G. Borovoi, E. I. Naats, “Scattering of light by a red blood cell,” J. Biomed. Opt. 3, 364–372 (1998). [CrossRef] [PubMed]
  10. A. Roggan, M. Friebe, K. Dörschel, A. Hahn, G. Müller, “Optical properties of circulating human blood,” in Laser–Tissue Interaction, Tissue Optics, and Laser Welding, G. P. Delacretaz, L. O. Svaasand, R. W. Steiner, R. Pini, G. Godlewski, eds., Proc. SPIE3195, 51–63 (1998). [CrossRef]
  11. A. N. Yaroslavsky, I. V. Yaroslavsky, T. Goldbach, H.-J. Schwarzmaier, “The optical properties of blood in the near infrared spectral range,” in Optical Diagnostics of Living Cells and Biofluids, D. L. Farkas, R. C. Leif, A. V. Priezzhev, T. Asakura, B. J. Tromberg, eds., Proc. SPIE2678, 314–324 (1996). [CrossRef]
  12. A. N. Yaroslavsky, I. V. Yaroslavsky, T. Goldbach, H.-J. Schwarzmaier, “Different phase function approximation to determine optical properties of blood: a comparison,” in Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, A. V. Priezzhev, T. Asakura, R. C. Leif, eds., Proc. SPIE2982, 324–330 (1997). [CrossRef]
  13. A. Ishimaru, Wave Propagation and Scattering in Random Media (Academic, New York, 1978).
  14. F. G. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, New York, 1983).
  15. B. Michel, “Statistical method to calculate extinction by small irregularly shaped particles,” J. Opt. Soc. Am. A 12, 2471–2481 (1995). [CrossRef]
  16. L. G. Henyey, J. L. Greenstein, “Diffuse radiation in the galaxy,” Astrophys. J. 93, 70–83 (1941). [CrossRef]
  17. L. O. Reynolds, N. J. McCormick, “Approximate two parameter phase function for light scattering,” J. Opt. Soc. Am. 70, 1206–1212 (1980). [CrossRef]
  18. M. M. Wintrobe, Clinical Hematology (Lea and Febiger, Philadelphia, 1981).
  19. R. Barer, S. Joseph, “Refractometry of living cells,” Q. J. Microsc. Sci. 95, 399–406 (1954).
  20. R. B. Pennell, “Composition of normal human red cells,” in The Red Blood Cell, C. Bishop, D. M. Surgenor, eds. (Academic, New York, 1964).
  21. G. Kortüm, Reflexionsspektroskopie (Springer-Verlag, Berlin, 1969). [CrossRef]
  22. O. W. van Assendelft, Spectrophotometry of Hemoglobin Derivatives (Roal Vangorcum, Assen, 1970).
  23. P. Latimer, “Anomalous dispersion of CS2 and CHCL3—theoretical predictions,” J. Opt. Soc. Am. 51, 116–118 (1961). [CrossRef]

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