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Applied Optics

Applied Optics


  • Vol. 37, Iss. 34 — Dec. 1, 1998
  • pp: 8103–8111

Mathematical model of fluorescence endoscopic image formation

Thomas D. Wang, G. Sargent Janes, Yang Wang, Irving Itzkan, Jacques Van Dam, and Michael S. Feld  »View Author Affiliations

Applied Optics, Vol. 37, Issue 34, pp. 8103-8111 (1998)

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We present a mathematical model that describes the spatial distribution of photons in fluorescence endoscopic images, resulting in expressions for image signal-to-noise ratio and resolution. This model was applied to quantitative analysis of fluorescence images collected from human colonic mucosa with a fiber-optic and an electronic endoscope. It provides a tool for the design of fluorescence endoscopic imaging systems and for extraction of quantitative information about image features. The results apply generally to endoscopic imaging of remote structures in biological and industrial settings, in which light of weak intensity such as fluorescence as well as reflected white light is used.

© 1998 Optical Society of America

OCIS Codes
(040.3780) Detectors : Low light level
(060.2350) Fiber optics and optical communications : Fiber optics imaging
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2680) Medical optics and biotechnology : Gastrointestinal
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(300.2530) Spectroscopy : Fluorescence, laser-induced

Original Manuscript: March 20, 1998
Revised Manuscript: August 4, 1998
Published: December 1, 1998

Thomas D. Wang, G. Sargent Janes, Yang Wang, Irving Itzkan, Jacques Van Dam, and Michael S. Feld, "Mathematical model of fluorescence endoscopic image formation," Appl. Opt. 37, 8103-8111 (1998)

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