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Applied Optics

Applied Optics


  • Vol. 37, Iss. 6 — Feb. 20, 1998
  • pp: 998–1005

On-column refractive-index detection based on retroreflected beam interference for capillary electrophoresis

Yanzhuo Deng and Bincheng Li  »View Author Affiliations

Applied Optics, Vol. 37, Issue 6, pp. 998-1005 (1998)

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A novel refractive-index detection scheme used for capillary electrophoresis detection, based on the interference of two retroreflected beams from the outer surface of a capillary tube illuminated by a focused laser beam, is described. A theoretical description and experimental measurement of the intensity profile of the interference fringe pattern in the detection plane are presented. The factors that limit the sensitivity of the proposed refractive-index detection scheme are discussed and compared with the transmitted beam interference and backscatter-based refractive-index detection configurations. The proposed refractive-index detector was used successfully for detection of capillary electrophoresis separations of saccharose, maltose, and lactose with a capillary tube of 50-μm inner diameter and a simple experimental setup.

© 1998 Optical Society of America

OCIS Codes
(040.1880) Detectors : Detection
(120.5710) Instrumentation, measurement, and metrology : Refraction
(260.3160) Physical optics : Interference

Original Manuscript: September 17, 1997
Published: February 20, 1998

Yanzhuo Deng and Bincheng Li, "On-column refractive-index detection based on retroreflected beam interference for capillary electrophoresis," Appl. Opt. 37, 998-1005 (1998)

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