OSA's Digital Library

Applied Optics

Applied Optics


  • Vol. 38, Iss. 1 — Jan. 1, 1999
  • pp: 230–235

Light-scattering properties of individual erythrocytes

Alexandr N. Shvalov, Juhani T. Soini, Andrey V. Chernyshev, Peter A. Tarasov, Erkki Soini, and Valeri P. Maltsev  »View Author Affiliations

Applied Optics, Vol. 38, Issue 1, pp. 230-235 (1999)

View Full Text Article

Enhanced HTML    Acrobat PDF (432 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We have studied the light-scattering properties of human erythrocytes both experimentally and theoretically. In the experimental study measurements were performed with a Scanning Flow Cytometer (SFC). The SFC can measure the light-scattering pattern (indicatrix) of an individual particle over an angular range of 10–60°. We have observed polymorphism in the measured set of indicatrices. To understand the reason for this polymorphism, we have made a theoretical study of the scattering properties of erythrocytes. The Wentzel–Kramer–Brillouin approximation has been employed to calculate indicatrices of individual erythrocytes in different orientations relative to the incident light beam. The indicatrices were calculated over an angular range of 15–35°. A comparison of the experimentally measured and theoretically calculated indicatrices shows that the polymorphism is due mainly to the different orientation of the erythrocytes in the flow. The effect caused by the Poiseuille profile of the flow on an individual erythrocyte within the SFC cuvette capillary was studied theoretically by use of the Stokes approximation. Rotation of an erythrocyte was predicted by this theoretical analysis, and this prediction was further verified by comparison with experimental results.

© 1999 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(290.5850) Scattering : Scattering, particles

Original Manuscript: February 10, 1998
Revised Manuscript: September 4, 1998
Published: January 1, 1999

Alexandr N. Shvalov, Juhani T. Soini, Andrey V. Chernyshev, Peter A. Tarasov, Erkki Soini, and Valeri P. Maltsev, "Light-scattering properties of individual erythrocytes," Appl. Opt. 38, 230-235 (1999)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. A. V. Chernyshev, V. I. Prots, A. A. Doroshkin, V. P. Maltsev, “Measurement of scattering properties of individual particles with a scanning flow cytometer,” Appl. Opt. 34, 6301–6305 (1995). [CrossRef] [PubMed]
  2. J. T. Soini, A. V. Chernyshev, P. E. Hanninen, E. Soini, V. P. Maltsev, “A new design of the flow cuvette and optical setup for the Scanning Flow Cytometer,” Cytometry 31, 78–84 (1998). [CrossRef] [PubMed]
  3. H. C. van de Hulst, Light Scattering by Small Particles (Wiley, New York, 1957).
  4. J. D. Klett, R. A. Sutherland, “Approximate methods for modeling the scattering properties of nonspherical particles: evaluation of the Wentzel–Kramers–Brillouin method,” Appl. Opt. 31, 373–386 (1992). [CrossRef] [PubMed]
  5. G. J. Steekstra, A. G. Hoeksta, E. Nijhof, R. M. Heethaar, “Light scattering by red blood cells in ektacytometry: Fraunhofer versus anomalous diffraction,” Appl. Opt. 32, 2266–2272 (1993). [CrossRef]
  6. V. N. Lopatin, N. V. Shepelevich, “Consequences of the integral wave equation in the Wentsel–Kramers–Brilluen approximation,” Opt. Spectrosc. 81, 103–106 (1996).
  7. P. Barber, C. Yeh, “Scattering of electromagnetic waves by arbitrarily shaped dielectric bodies,” Appl. Opt. 14, 2864–2872 (1975). [CrossRef] [PubMed]
  8. A. M. K. Nilsson, P. Alsholm, A. Karlsson, S. Andersson-Engels, “T-matrix computations of light scattering by red blood cells,” Appl. Opt. 37, 2735–2748 (1998). [CrossRef]
  9. N. K. Uzunoglu, D. Yova, G. S. Stamatakos, “Light scattering by pathological and deformed erythrocytes: an integral equation model,” J. Biomed. Opt. 2, 310–318 (1997). [CrossRef]
  10. Y. C. Fung, W. C. O. Tsang, P. Patitucci, “High-resolution data on the geometry of red blood cells,” Biorheology 18, 369–385 (1981). [PubMed]
  11. D. H. Tycko, M. H. Metz, E. A. Epstein, A. Grinbaum, “Flow-cytometric light scattering measurement of red blood cell volume and hemoglobin concentration,” Appl. Opt. 24, 1355–1365 (1985). [CrossRef] [PubMed]
  12. P. Mazeron, S. Muller, H. El. Azouzi, “On intensity reinforcements in small-angle light scattering patterns of erythrocytes under shear,” Eur. Biophys. J. 26, 247–252 (1997).
  13. P. Mazeron, S. Muller, H. El. Azouzi, “Deformation of erythrocytes under shear: a small-angle light scattering study,” Biorheology34, 99–110 (1997). [PubMed]
  14. R. Skalak, A. Tozeren, R. P. Zarda, S. Chien, “Strain energy function of red blood cell membranes,” Biophys. J. 13, 245–264 (1973). [CrossRef] [PubMed]
  15. V. P. Maltsev, A. V. Chernyshev, K. A. Semyanov, E. Soini, “Absolute real-time measurement of particle size distribution with the method of flying light scattering indicatrix,” Appl. Opt. 35, 3275–3280 (1996). [CrossRef] [PubMed]
  16. C. F. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, New York, 1983).
  17. H. Brenner, “The Stokes resistance of an arbitrary particle,” Chem. Eng. Sci. 18, 1–25 (1963). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited