Measurement of absorption and reduced-scattering optical coefficients μ<sub><i>a</i></sub> and μ<sub><i>s</i></sub>′ is possible when a steady-state backscattered image is used on a sample surface. A new method for processing the backscattered image, acquired with a CCD, has been developed. The image is integrated to decrease sensitivity to noise. The resulting curve is defined as the integral reflectance. The curve is then fitted with a relaxation model to evaluate μ<sub><i>a</i></sub> and μ<sub><i>s</i></sub>′. We have validated the method with calibrated scattering and absorption phantoms. The integral reflectance method is then applied to measurements of the μ<sub><i>a</i></sub> and μ<sub><i>s</i></sub>′ coefficients of human skin <i>in vivo</i>.
© 1999 Optical Society of America
Laure Gobin, Loïc Blanchot, and Hervé Saint-Jalmes, "Integrating the Digitized Backscattered Image to Measure Absorption and Reduced-Scattering Coefficients in vivo," Appl. Opt. 38, 4217-4227 (1999)