OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 38, Iss. 34 — Dec. 1, 1999
  • pp: 7133–7144

Slit-Scanning Confocal Microendoscope for High-Resolution In Vivo Imaging

Yashvinder S. Sabharwal, Andrew R. Rouse, LaTanya Donaldson, Mark F. Hopkins, and Arthur F. Gmitro  »View Author Affiliations


Applied Optics, Vol. 38, Issue 34, pp. 7133-7144 (1999)
http://dx.doi.org/10.1364/AO.38.007133


View Full Text Article

Acrobat PDF (684 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We discuss the design and construction of a novel imaging system in which a fiber-optic imaging bundle and miniature optical and mechanical components are used to allow confocal fluorescence microscopy in remote locations. The instrumentation has been developed specifically for cellular examination of tissue for optical biopsy. Miniaturization of various components makes the device usable in a clinical setting. The numerical aperture of the beam in the tissue is 0.5, and the field of view is 430 μm. The measured lateral resolution of the system is 3.0 μm. The axial point and the axial planar response functions of the confocal system were measured with a FWHM of 10 and 25 μm, respectively. In vitro and in vivo images obtained with cell cultures, human tissue specimens, and animal models indicate that the performance of the device is adequate for microscopic evaluation of cells.

© 1999 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

Citation
Yashvinder S. Sabharwal, Andrew R. Rouse, LaTanya Donaldson, Mark F. Hopkins, and Arthur F. Gmitro, "Slit-Scanning Confocal Microendoscope for High-Resolution In Vivo Imaging," Appl. Opt. 38, 7133-7144 (1999)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-38-34-7133


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. M. Rajadhyaksha, R. G. B. Langley, S. Gonzales, M. White, J. M. Zavislan, R. H. Webb, and R. R. Anderson, “Clinical real-time confocal imaging of human skin and oral tissues in vivo,” in Conference Proceedings of LEOS ’97, 10th Annual Meeting of the IEEE Lasers and Electro-Optics Society (IEEE, N.Y., 1997).
  2. M. Hammer, D. Schweitzer, L. Leistritz, M. Scibor, K. Donnerhacke, and J. Strobel, “Imaging spectroscopy of human ocular fundus in vivo,” J. Biomed. Opt. 2, 418–425 (1997).
  3. B. R. Masters, “Three-dimensional confocal microscopy of the living in situ rabbit cornea,” Opt. Express 3, 59–63 (1998), http://epubs.osa.org/opticsexpress.
  4. A. F. Gmitro and D. Aziz, “Confocal microscopy through a fiber-optic imaging bundle,” Opt. Lett. 18, 565–567 (1993).
  5. R. Juskaitis, T. Wilson, and T. F. Watson, “Real-time white light reflection confocal microscopy using a fibre-optic bundle,” Scanning 19, 15–19 (1997).
  6. T. Collier, C. Smithpeter, B. Cowman, R. Drezek, M. Bescour, and R. Richards-Kortum, “Fiber-optic confocal microscope for biological imaging,” in Conference on Lasers and Electro-Optics, Vol. 6 of 1998 Technical Digest Series (Optical Society of America, Washington, D.C., 1998), pp. 128–129.
  7. D. L. Dickensheets and G. S. Kino, “Silicon-micromachined scanning confocal optical microscope,” J. Microelectromech. Syst. 7, 38–47 (1998).
  8. M. Minsky, “Microscopy apparatus,” U.S. patent 3,013,467 (1961).
  9. D. Aziz, “Confocal microscopy through a fiber-optic imaging bundle,” Ph.D. dissertation (Optical Sciences Library, The University of Arizona, Tucson, Ariz., 1994), pp. 25–30.
  10. Y. Sabharwal, “Remote-access slit-scanning confocal microscope for in vivo tumor diagnosis,” Ph.D. dissertation (Optical Sciences Library, The University of Arizona, Tucson, Ariz., 1998), pp. 50–51.
  11. T. Wilson, Confocal Microscopy (Academic, London, 1990), pp. 114–118.
  12. M. A. Player, “Spread functions and modulation transfer functions of fibre-optic bundles,” J. Mod. Opt. 35, 1363–1372 (1988).
  13. Y. Sabharwal, “Remote-access slit-scanning confocal microscope for in vivo tumor diagnosis,” Ph.D. dissertation (Optical Sciences Library, (The University of Arizona, Tucson, Ariz., 1998), pp. 51–54.
  14. J. D. Gaskill, Linear Systems, Fourier Transforms, and Optics (Wiley, New York, 1978), pp. 339–345.
  15. R. P. Haugland, Handbook of Fluorescent Probes and Research Chemicals (Molecular Probes, Inc., Eugene, Oreg., 1996), pp. 148–149.
  16. A. R. Parrish, A. J. Gandolfi, and K. Brendel, “Precision-cut tissue slices: applications in pharmacology and toxicology,” Life Sci. 57, 1887–1901 (1995).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited