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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 39, Iss. 19 — Jul. 1, 2000
  • pp: 3344–3356

Excitation-and-collection geometry insensitive fluorescence imaging of tissue-simulating turbid media

Jianan Y. Qu, Zhijian Huang, and Jianwen Hua  »View Author Affiliations


Applied Optics, Vol. 39, Issue 19, pp. 3344-3356 (2000)
http://dx.doi.org/10.1364/AO.39.003344


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Abstract

We present an imaging technique for the correction of geometrical effects in fluorescence measurement of optically thick, turbid media such as human tissue. Specifically, we use the cross-polarization method to reject specular reflection and enhance the diffusive backscattering of polarized fluorescence excitation light from the turbid media. We correct the nonuniformity of the image field caused by the excitation-and-collection geometry of a fluorescence imaging system by normalizing the fluorescence image to the cross-polarized reflection image. The ratio image provides a map of relative fluorescence yield, defined as the ratio of emerging fluorescence power to incident excitation, over the surface of an imaged homogeneous turbid medium when fluorescence excitation-and-collection geometries vary in a wide range. We investigate the mechanism of ratio imaging by using Monte Carlo modeling. Our findings show that this technique could have a potential use in the detection of early cancer, which usually starts from a superficial layer of tissue, based on the contrast in the tissue fluorescence of an early lesion and of the surrounding normal tissue.

© 2000 Optical Society of America

OCIS Codes
(110.7050) Imaging systems : Turbid media
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

History
Original Manuscript: June 7, 1999
Revised Manuscript: February 16, 2000
Published: July 1, 2000

Citation
Jianan Y. Qu, Zhijian Huang, and Jianwen Hua, "Excitation-and-collection geometry insensitive fluorescence imaging of tissue-simulating turbid media," Appl. Opt. 39, 3344-3356 (2000)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-39-19-3344

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