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Applied Optics

Applied Optics


  • Vol. 39, Iss. 19 — Jul. 1, 2000
  • pp: 3388–3395

Temperature-related reversible birefringence changes in rat tail tendon

Didier Beghuin, Klaus Schönenberger, Guy Delacrétaz, and René Paul Salathé  »View Author Affiliations

Applied Optics, Vol. 39, Issue 19, pp. 3388-3395 (2000)

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By use of a highly sensitive method for measuring slight variations in birefringence it is shown here that a strong reversible correlation exists between rat tail tendon birefringence and temperature. This phenomenon is totally different from the loss of birefringence that results from a denaturation process. Below the threshold temperature leading to denaturation, an increase in temperature is systematically accompanied by a reversible increase in birefringence (0.25% °C-1). This phenomenon is observed at very fast heating rates (250,000 °C s-1), such as those induced by pulsed infrared lasers, and confirmed by experiments conducted with slow homogeneous heating of the sample medium (0.1 °C s-1). The good correlation between birefringence and temperature observed during the fast heating suggests that there are only small modifications of the tissue structure at the fibril level.

© 2000 Optical Society of America

OCIS Codes
(120.5410) Instrumentation, measurement, and metrology : Polarimetry
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(260.1440) Physical optics : Birefringence
(350.5340) Other areas of optics : Photothermal effects

Original Manuscript: October 26, 1999
Revised Manuscript: March 30, 2000
Published: July 1, 2000

Didier Beghuin, Klaus Schönenberger, Guy Delacrétaz, and René Paul Salathé, "Temperature-related reversible birefringence changes in rat tail tendon," Appl. Opt. 39, 3388-3395 (2000)

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