OSA's Digital Library

Applied Optics

Applied Optics


  • Vol. 39, Iss. 19 — Jul. 1, 2000
  • pp: 3408–3412

Fiber-optic microsensors to measure backscattered light intensity in biofilms

Haluk Beyenal, Zbigniew Lewandowski, Chris Yakymyshyn, Brad Lemley, and Joe Wehri  »View Author Affiliations

Applied Optics, Vol. 39, Issue 19, pp. 3408-3412 (2000)

View Full Text Article

Enhanced HTML    Acrobat PDF (121 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We have constructed tapered fiber-optic microsensors with a tip diameter of less than 10 µm to measure profiles of backscattered light in biofilms, which are thin layers of micro-organisms firmly attached to surfaces. The observed response agrees well with local effective diffusivity microelectrode measurements, with R2 > 0.85. A strong relation between signal intensity and wavelength has been observed at 670 and 1320 nm. These sensors have the potential to replace local effective diffusivity microelectrodes for true in situ biofilm measurements.

© 2000 Optical Society of America

OCIS Codes
(290.1350) Scattering : Backscattering
(290.1990) Scattering : Diffusion

Original Manuscript: November 29, 1999
Revised Manuscript: March 27, 2000
Published: July 1, 2000

Haluk Beyenal, Zbigniew Lewandowski, Chris Yakymyshyn, Brad Lemley, and Joe Wehri, "Fiber-optic microsensors to measure backscattered light intensity in biofilms," Appl. Opt. 39, 3408-3412 (2000)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. J. W. Costerton, Z. Lewandowski, D. E. Caldwell, D. R. Korber, H. M. Lappin-Scott, “Microbial biofilms,” Annu. Rev. Microbiol. 49, 711–745 (1995). [CrossRef] [PubMed]
  2. A. B. Cunningham, B. M. Peyton, “Bench-scale/field-scale interpretations: an Overview,” in Applied Bioremediation of Petroleum Hydrocarbons, R. E. Hinchee, J. A. Kittel, H. J. Reisinger, eds. (Battelle, Dayton, Ohio, 1995), pp. 97–100.
  3. P. D. Marsh, “Dental plaque,” in Microbial Biofilms, H. M. Lappin-Scott, J. W. Costerton, eds., (Cambridge U. Press, New York, 1995), pp. 282–300. [CrossRef]
  4. C. I. Chen, M. A. Reinsel, R. F. Mueller, “Kinetic investigation of microbial souring in porous media using microbial consortia from oil reservoirs,” Biotechnol. Bioeng. 44, 263–269 (1994). [CrossRef] [PubMed]
  5. W. Lee, Z. Lewandowski, P. H. Nielsen, W. A. Hamilton, “Role of sulfate-reducing bacteria in corrosion of mild steel: a review,” Biofouling 8, 165–194 (1995). [CrossRef]
  6. A. G. Gristina, “Biofilms and chronic bacterial infections,” Clin. Microbiol. News. 16, 171–178 (1994). [CrossRef]
  7. N. P. Revsbech, “An oxygen microsensor with a guard cathode,” Limnol. Oceanogr. 34, 474–478 (1989). [CrossRef]
  8. S. Yang, Z. Lewandowski, “Measurement of local mass transfer coefficient in biofilms,” Biotechnol. Bioeng. 48, 737–744 (1995). [CrossRef] [PubMed]
  9. H. Beyenal, A. Tanyolaç, Z. Lewandowski, “Measurement of local effective diffusivity and cell density variations in heterogeneous biofilms,” Water Sci. Technol. 38, 171–178 (1998). [CrossRef]
  10. I. Klimant, G. Holst, M. Kuhl, “A simple fiber optic sensor to detect the penetration of microsensors in the sediments and other biogeochemical systems,” Limnol. Oceanogr. 42, 1638–1643 (1997). [CrossRef]
  11. H. Gao, Z. Chen, J. Kumar, S. Tripathy, D. Kaplan, “Tapered fiber tips for fiber optic biosensors,” Opt. Eng. 34, 3465–3470 (1995). [CrossRef]
  12. M. Born, E. Wolf, Principles of Optics, 6th ed. (Pergamon, Oxford, UK, 1980).
  13. M. Yarborough, Photonics Design and Applications Handbook (Laurin, Pittsfield, Mass., 1996), pp. H287–H290.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4 Fig. 5

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited