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Applied Optics

Applied Optics


  • Vol. 39, Iss. 21 — Jul. 20, 2000
  • pp: 3738–3745

Simultaneous light scattering and intrinsic fluorescence measurement for the classification of airborne particles

Paul H. Kaye, John E. Barton, Edwin Hirst, and James M. Clark  »View Author Affiliations

Applied Optics, Vol. 39, Issue 21, pp. 3738-3745 (2000)

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We describe a prototype laboratory light-scattering instrument that integrates two approaches to airborne particle characterization: spatial light-scattering analysis and intrinsic fluorescence measurement, with the aim of providing an effective means of classifying biological particles within an ambient aerosol. The system uses a single continuous-wave 266-nm ultraviolet laser to generate both the spatial elastic scatter data (from which an assessment of particle size and shape is made) and the particle intrinsic fluorescence data from particles in the approximate size range of 1–10-µm diameter carried in a sample airflow through the laser beam. Preliminary results suggest that this multiparameter measurement approach can provide an effective means of classifying different particle types and can reduce occurrences of false-positive detection of biological aerosols.

© 2000 Optical Society of America

OCIS Codes
(120.1880) Instrumentation, measurement, and metrology : Detection
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(290.1090) Scattering : Aerosol and cloud effects
(290.5820) Scattering : Scattering measurements
(300.2530) Spectroscopy : Fluorescence, laser-induced

Original Manuscript: November 22, 1999
Revised Manuscript: April 4, 2000
Published: July 20, 2000

Paul H. Kaye, John E. Barton, Edwin Hirst, and James M. Clark, "Simultaneous light scattering and intrinsic fluorescence measurement for the classification of airborne particles," Appl. Opt. 39, 3738-3745 (2000)

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